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KMID : 0811719990030010093
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Korean Journal of Physiology & Pharmacology
1999 Volume.3 No. 1 p.93 ~ p.100
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Role of Phospholipase A2 in Hypoxia-Induced Renal Cell Injury
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Won Rak Choi
Sun Hee Ko/Su In Cho/Jae Suk Woo/Jin Sup Jung/Sang Ho Lee/Yong Keun Kim
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Abstract
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The present study was designed to assess the roles of PLA2 activation and arachidonic acid (AA) metabolites in hypoxia-induced renal cell injury. Hypoxia increased LDH release in a dose-dependent manner in rabbit renal cortical slices, and this increase was significant after 20-min hypoxia. The hypoxia-induced LDH release was prevented by amino acids, glycine and alanine, and extracellular acidosis (pH 6.0). Buffering intracellular Ca2+ by a chelator, but not omission of Ca2+ in the medium produced a significant reduction in hypoxia-induced LDH release. The effect of hypoxia was blocked by PLA2 inhibitors, mepacrine, butacaine, and dibucaine. A similar effect was observed by a 85-kD cPLA2 inhibitor AACOCF3. AA increased hypoxia-induced LDH release, and albumin, a fatty acid absorbent, prevented the LDH release, suggesting that free fatty acids are involved in hypoxia-induced cell injury. These results suggest that PLA2 activation and its metabolic products play important roles in pathogenesis of hypoxia-induced cell injury in rabbit renal cortical slices.
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KEYWORD
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Oxidant, PLA2, LDH release, Hypoxia, Rabbit kidney,
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